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KMID : 0613820220320030189
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Journal of Life Science
2022 Volume.32 No. 3 p.189 ~ p.195
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Brain-expressed X-linked 2 Binds to Kinesin Superfamily Protein 3A
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Kim Moo-Seong
Jeong Young-Joo
Park Sung-Woo
Seo Mi-Kyoung
Kim Sang-Jin
Lee Won-Hee
Urm Sang-Hwa
Lee Jung-Goo
Seog Dae-Hyun
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Abstract
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Kinesin-2 comprises two subfamilies of the heterotrimeric or homodimeric motors found in mammalian cells. Heterotrimeric kinesin-2 consists of kinesin superfamily proteins (KIFs) 3A and 3B and kinesin-associated protein 3 (KAP3), which is a molecular motor protein that moves along microtubules. It plays diverse roles in cargo transport, including anterograde trafficking in cilia, and interacts with many different cargoes and proteins, but their binding proteins have not yet been fully identified. In this study, the yeast two-hybrid assay was used to identify the proteins that interact with the cargo-binding domain (CBD) of KIF3A, and an interaction between KIF3A and brain expressed X-linked 2 (Bex2) was found. Bex2 bound to the CBD-containing C-terminal tail region of KIF3A but did not interact with the same region of KIF3B or KIF5A (a motor protein of kinesin-1). KIF3A interacted with another isoform, Bex1, but did not interact with Bex3. In addition, glutathione S-transferase (GST) pull-downs showed that KIF3A specifically interacts with GST-Bex1 and GST-Bex2 but not with GST alone. When co-expressed in HEK-293T cells, Bex2 co-localized with KIF3A and co-immunoprecipitated with KIF3A and KIF3B but not KIF5B. In combination, these results suggest that Bex2 is capable of binding to heterotrimeric kinesin-2 and may serve as an adaptor protein that links heterotrimeric kinesin-2 with cargo.
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KEYWORD
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Binding protein, brain expressed X-linked 2, cargo binding domain, kinesin, heterotrimeric kinesin-2
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